growth rate  (ATCC)

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Young adult control and Kdm5 LOF flies (5–7 days old) were treated with 5% sucrose (mock control), Lactiplantibacillus plantarum (Lp), Lactobacillus helveticus (Lh), or a 1:1 combination of Lp and Lh in 5% sucrose for 2–3 days, supplemented on standard fly food. Guts were dissected, homogenized and plated on selective agar media to quantify CFUs per gut of Acetobacter (A) , Enterobacter (B) and Lactobacilli (C) genera. Bars represent mean ± SEM from three independent biological replicates, with duplicate technical platings per sample. Statistical significance was determined by one-way ANOVA followed by Dunnetts multiple-comparisons test. **p < 0.01; ****p < 0.0001; ns, not significant.

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Young adult control and Kdm5 LOF flies (5–7 days old) were treated with 5% sucrose (mock control), Lactiplantibacillus plantarum (Lp), Lactobacillus helveticus (Lh), or a 1:1 combination of Lp and Lh in 5% sucrose for 2–3 days, prior to behavioral testing. Social spacing behavior was assessed using the Social Distance assay. Histograms (A–H) show the percentage of flies occupying 0.5-cm distance bins, calculated based on the distance from the nearest to the farthest neighbor. Panels A–D correspond to control flies, and panels E–H correspond to Kdm5 LOF flies under the indicated treatment conditions. (I) The Social Space Index (SSI) was calculated as the percentage of flies in the first distance bin minus the percentage in the second bin for each condition. Positive SSI values indicate increased social proximity, whereas values at or below zero indicate reduced social interaction. Bars represent mean ± SEM from three independent biological replicates. Statistical significance was determined using a two-way ANOVA followed by Šídák’s multiple-comparisons test. All post hoc comparisons were performed within genotypes; only the two statistically significant comparisons are indicated (**p < 0.01; ***p < 0.001).

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Young adult control (gut-specific Lucierase RNAi ) and gut-specific Kdm5 RNAi flies (5-7 days old; RNAi induced at 29°C for 3 days) were treated with 5% sucrose (mock control), L. plantarum ( Lp ), L. helveticus ( Lh ) or a 1:1 combination of Lp and Lh in 5% sucrose for 2-3 days, supplemented on standard fly food. Guts were dissected, homogenized and plated on selective agar media to quantify CFUs per gut of Acetobacter (A) , Enterobacter (B) and Lactobacilli (C) genera. Bars represent mean ± SEM from three independent biological replicates, with duplicate technical platings per sample. Statistical significance was determined by one-way ANOVA followed by Dunnett’s or Šídák’s multiple-comparisons test, as indicated. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Young adult control (gut-specific Lucierase RNAi ) and gut-specific Kdm5 RNAi flies (5-7 days old; (5-7 days old; RNAi induced at 29°C for 3 days) were treated with 5% sucrose (mock control), L. plantarum ( Lp ), L. helveticus ( Lh ) or a 1:1 combination of Lp and Lh in 5% sucrose for 2-3 days prior to behavioral testing. Social spacing behavior was assessed using the social distance assay. Histograms (A–H) show the percentage of flies occupying 0.5-cm distance bins, calculated based on the distance from each fly to its nearest neighbor. Panels A–D correspond to control flies, and panels E–H correspond to gut-specific Kdm5 RNAi flies under the indicated treatment conditions. (I) The Social Space Index (SSI) was calculated as the percentage of flies in the first distance bin minus the percentage in the second bin for each condition. Positive SSI values indicate increased social proximity, whereas SSI values ≤ 0 indicate reduced social interaction. Bars represent mean ± SEM from three independent biological replicates. Statistical significance was determined using a two-way ANOVA followed by Šídák’s multiple-comparisons test; no post hoc SSI comparisons reached statistical significance.

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Journal: bioRxiv

Article Title: Microbiota-Based Interventions Differentially Rescue Gut and Social Behavior Phenotypes in a Drosophila Autism-like Model

doi: 10.64898/2026.01.09.698713

Figure Lengend Snippet: Differential abundance analysis was performed on 16S rRNA gene sequencing data from recipient flies following FMT. (A) Coefficient plot showing differential abundance of selected bacterial taxa across donor–recipient combinations following FMT. Positive coefficient values indicate relative enrichment, whereas negative values indicate relative depletion. (B–C) Relative abundance of Lactobacillus plantarum (B) and Lactobacillus pentosus–plantarum (C) in control and Kdm5 LOF recipient flies following FMT. Individual points represent biological replicates. Each condition represents three independent biological replicates. Statistical significance was determined using differential abundance analysis with false discovery rate (FDR) correction. Adjusted p-values and coefficients are indicated where significant.

Article Snippet: The probiotics used were Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum ) (ATCC 14917) and Lactobacillus helveticus (ATCC 15009), both obtained from the American Type Culture Collection (ATCC).

Techniques: Sequencing, Control